Image cytometry: nuclear and chromosomal DNA quantification.

  title={Image cytometry: nuclear and chromosomal DNA quantification.},
  author={Carlos Roberto Carvalho and Wellington Ronildo Clarindo and Isabella Santiago de Abreu},
  journal={Methods in molecular biology},
Image cytometry (ICM) associates microscopy, digital image and software technologies, and has been particularly useful in spatial and densitometric cytological analyses, such as DNA ploidy and DNA content measurements. Basically, ICM integrates methodologies of optical microscopy calibration, standard density filters, digital CCD camera, and image analysis softwares for quantitative applications. Apart from all system calibration and setup, cytological protocols must provide good slide… CONTINUE READING

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