Image-based transcriptomics in thousands of single human cells at single-molecule resolution

  title={Image-based transcriptomics in thousands of single human cells at single-molecule resolution},
  author={Nico Battich and Thomas Stoeger and Lucas Pelkmans},
  journal={Nature Methods},
Fluorescence in situ hybridization (FISH) is widely used to obtain information about transcript copy number and subcellular localization in single cells. However, current approaches do not readily scale to the analysis of whole transcriptomes. Here we show that branched DNA technology combined with automated liquid handling, high-content imaging and quantitative image analysis allows highly reproducible quantification of transcript abundance in thousands of single cells at single-molecule… 

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