Image analysis for automatic segmentation of cytoplasms and classification of Rac1 activation.

@article{Lindblad2004ImageAF,
  title={Image analysis for automatic segmentation of cytoplasms and classification of Rac1 activation.},
  author={Joakim Lindblad and Carolina W{\"a}hlby and Ewert Bengtsson and Alla Borisovna Zaltsman},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2004},
  volume={57 1},
  pages={22-33}
}
BACKGROUND Rac1 is a GTP-binding molecule involved in a wide range of cellular processes. Using digital image analysis, agonist-induced translocation of green fluorescent protein (GFP) Rac1 to the cellular membrane can be estimated quantitatively for individual cells. METHODS A fully automatic image analysis method for cell segmentation, feature extraction, and classification of cells according to their activation, i.e., GFP-Rac1 translocation and ruffle formation at stimuli, is described… CONTINUE READING