Image analysis and mathematical correlation between cell number and diameter of colonies in soft agar as a measurement of growth in soft agar.


Automated image analysis was used to measure the colony diameter and estimate the cell number in colonies under conditions for anchorage-independent growth (AIG). The calculated values for the number of AIG cells in a colony of a diameter greater than or equal to 60 microns were decidedly different from the actual values obtained by either automated or… (More)


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