Image Analysis of Transient Expression in Bombarded Soybean ( Glycine max ) Immature Embryos

Particle bombardment of cells traditionally results in low frequencies of regenerated transgenic plants, which could be due to low (1-2%) cell viability in particle containing cells (Hunold, Bronner, & Hahne, 1994). Several methods for reducing damage to cells during bombardment have been developed, including the use of osmotic treatments (Vain, McMullen… CONTINUE READING