Ignored sediment fungal populations in water supply reservoirs are revealed by quantitative PCR and 454 pyrosequencing

  title={Ignored sediment fungal populations in water supply reservoirs are revealed by quantitative PCR and 454 pyrosequencing},
  author={H. Zhang and Tinglin Huang and S. Chen},
  journal={BMC Microbiology},
  • H. Zhang, Tinglin Huang, S. Chen
  • Published 2015
  • Biology, Medicine
  • BMC Microbiology
  • BackgroundThe sediment hosts a variety of fungal species in water supply reservoirs; however, the taxonomically and functionally diverse fungal populations have remained vastly unexplored. Here, quantitative PCR (qPCR) and recently developed high-throughput 454 GS FLX pyrosequencing were combined to investigate the abundance and diversity of sediment fungal communities in three water supply reservoirs.ResultsThese results revealed 1991, 2473, and 2610 copies of the 18S rRNA gene in the… CONTINUE READING
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