IgE-mediated release of histamine from human cutaneous mast cells.

  title={IgE-mediated release of histamine from human cutaneous mast cells.},
  author={Michael D. Tharp and R T Suvunrungsi and Timothy John Sullivan},
  journal={Journal of immunology},
  volume={130 4},
We investigated the ability of antigen-IgE interactions to stimulate histamine release from human infant cutaneous mast cells. Skin obtained at circumcision contained numerous perivascular mast cells, as assessed by light and electron microscopy. The histamine content of this tissue averaged 17.7 ng (+/- 1.5 SEM)/mg wet weight. Challenge of 200-microns thick sections of unsensitized skin with varying concentrations of monoclonal murine antibodies to human IgE caused no net release of histamine… CONTINUE READING