IgE-dependent expression of mRNA for IL-4 and IL-5 in human lung mast cells.

@article{Okayama1995IgEdependentEO,
  title={IgE-dependent expression of mRNA for IL-4 and IL-5 in human lung mast cells.},
  author={Yoko Okayama and Corinne Petit-Fr{\'e}re and Olivier Kassel and A E Semper and Douglas J. Quint and M J Tunon-de-Lara and Peter Bradding and Stephen T. Holgate and M K Church},
  journal={Journal of immunology},
  year={1995},
  volume={155 4},
  pages={1796-808}
}
By using the reverse transcriptase (RT)-PCR and in situ hybridization we have studied the expression of mRNA for IL-5 and IL-4 in human lung mast cells induced by cross-linkage of high affinity Fc epsilon Rs. Lung mast cells were purified using affinity magnetic selection with mAb YB5.B8 against c-kit to achieve a final mast cell purity > 93%. Purified mast cells were precultured with stem cell factor (SCF) (10 ng/ml) and myeloma IgE (3 micrograms/ml) for 16 h before challenge with anti-IgE (1… CONTINUE READING