Identifying translationally regulated genes during stem cell differentiation.

@article{Sampath2011IdentifyingTR,
  title={Identifying translationally regulated genes during stem cell differentiation.},
  author={Prabha Sampath and Qian Yi Lee and Vivek Tanavde},
  journal={Current protocols in stem cell biology},
  year={2011},
  volume={Chapter 1},
  pages={Unit1B.8}
}
This unit describes a protocol for genome-wide identification of translationally regulated genes during embryonic stem cell differentiation using integrated transcriptome and translation state profiling. Actively translated mRNAs associated with multiple ribosomes (known as polysomes) and translationally inactive mRNAs sequestered in messenger ribonucleoprotein particles (mRNPs), can be separated by sucrose gradient fractionation based on size. Because the number of ribosomes on a transcript… CONTINUE READING

References

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Integrating transcriptome and translational state profiling to identify genes under translational control during embryonic stem cell differentiation

  • Q. Y. Lee, W. Koh, P. Sampath, V. Tanavde
  • Computational Biology of Embryonic Stem Cells
  • 2011
2 Excerpts

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