Identifying key cereal aphid predators by molecular gut analysis.

@article{Chen2000IdentifyingKC,
  title={Identifying key cereal aphid predators by molecular gut analysis.},
  author={Yue Chen and Kristopher L. Giles and Mark Edward Payton and Matthew H. Greenstone},
  journal={Molecular ecology},
  year={2000},
  volume={9 11},
  pages={
          1887-98
        }
}
We describe polymerase chain reaction (PCR) primers for gut analysis of aphid predators. The primers amplify aphid mitochondrial COII fragments ranging in size from 77 to 386 bp. Using these primers, we were able to distinguish six species of US Great Plains cereal aphids, including two congeners, Rhopalosiphum maidis (Fitch) and R. padi (L.), and to detect them in extracts of coccinellid and chrysopid predators. We devised a protocol for deriving half-lives of detectability for the DNA of a… CONTINUE READING

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