Identifying differences in mRNA expression by representational difference analysis of cDNA.

  title={Identifying differences in mRNA expression by representational difference analysis of cDNA.},
  author={Mike Hubank and David G Schatz},
  journal={Nucleic acids research},
  volume={22 25},
Detection of differentially regulated genes has been severely hampered by technical limitations. In an effort to overcome these problems, the PCR-coupled subtractive process of representational difference analysis (RDA) [Lisitsyn, N. et al. (1993) Science 259, 946-951] has been adapted for use with cDNA. In a model system, RAG-1 and RAG-2, the genes responsible for activating V(D)J recombination, were identified in a genomic transfectant by cDNA RDA in a small fraction of the time taken by… CONTINUE READING
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