Identifying axon guidance defects in the embryonic zebrafish brain.

@article{Devine2003IdentifyingAG,
  title={Identifying axon guidance defects in the embryonic zebrafish brain.},
  author={Christine A. Devine and B. Key},
  journal={Methods in cell science : an official journal of the Society for In Vitro Biology},
  year={2003},
  volume={25 1-2},
  pages={
          33-7
        }
}
  • C. Devine, B. Key
  • Published 2003
  • Biology
  • Methods in cell science : an official journal of the Society for In Vitro Biology
The method described here outlines a simple protocol to investigate the in vivo function of axon guidance molecules during the development of the embryonic zebrafish brain. By 24 hours postfertilization, a simple scaffold of axon tracts and commissures can be visualised in the brain using acetylated alpha-tubulin, a panaxonal marker that stains all axons. The highly stereotypical trajectory of axons in the embryonic zebrafish brain provides an ideal system in which to study the molecular… 
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