Identifying and quantifying proteolytic events and the natural N terminome by terminal amine isotopic labeling of substrates

@article{Kleifeld2011IdentifyingAQ,
  title={Identifying and quantifying proteolytic events and the natural N terminome by terminal amine isotopic labeling of substrates},
  author={Oded Kleifeld and Alain Doucet and Anna Prudova and Ulrich Keller and Magda Gioia and Jayachandran N Kizhakkedathu and Christopher M. Overall},
  journal={Nature Protocols},
  year={2011},
  volume={6},
  pages={1578-1611}
}
Analysis of the sequence and nature of protein N termini has many applications. Defining the termini of proteins for proteome annotation in the Human Proteome Project is of increasing importance. Terminomics analysis of protease cleavage sites in degradomics for substrate discovery is a key new application. Here we describe the step-by-step procedures for performing terminal amine isotopic labeling of substrates (TAILS), a 2- to 3-d (depending on method of labeling) high-throughput method to… CONTINUE READING