Identifying and characterizing Hfq-RNA interactions.

@article{Faner2013IdentifyingAC,
  title={Identifying and characterizing Hfq-RNA interactions.},
  author={Martha Audra Faner and Andrew L Feig},
  journal={Methods},
  year={2013},
  volume={63 2},
  pages={144-59}
}
To regulate stress responses and virulence, bacteria use small regulatory RNAs (sRNAs). These RNAs can up or down regulate target mRNAs through base pairing by influencing ribosomal access and RNA decay. A large class of these sRNAs, called trans-encoded sRNAs, requires the RNA binding protein Hfq to facilitate base pairing between the regulatory RNA and its target mRNA. The resulting network of regulation is best characterized in Escherichia coli and Salmonella typhimurium, but the importance… CONTINUE READING