Identifying a “pseudogene” for the mitochondrial DNA COI region of the corixid aquatic insect, Hesperocorixa distanti (Heteroptera, Corixidae)

@article{Yano2020IdentifyingA,
  title={Identifying a “pseudogene” for the mitochondrial DNA COI region of the corixid aquatic insect, Hesperocorixa distanti (Heteroptera, Corixidae)},
  author={Koki Yano and Masaki Takenaka and Toshimasa Mitamura and Koji Tojo},
  journal={Limnology},
  year={2020},
  volume={21},
  pages={319 - 325}
}
DNA barcoding has been actively used as a method for species identification, and it will become an increasingly important method in the future. However, DNA barcoding can occasionally encounter a major problem, namely “nuclear mitochondrial DNA pseudogenes (NUMTs)”. In this paper, we report the discovery of a pseudogene in the mitochondrial COI region from aquatic heteropterans. PCR with Folmer’s universal primer set produced two different lengths of DNA sequences: a standard 658 bp sequence… 

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