Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins

@article{Lam2020IdentifyingSC,
  title={Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins},
  author={Tommy Tsan-Yuk Lam and Na Jia and Yawei Zhang and Marcus Ho-Hin Shum and Jia-Fu Jiang and Hua Chen Zhu and Yi-Gang Tong and Yongxia Shi and Xue-bing Ni and Yunshi Liao and Wen-Juan Li and Bao-Gui Jiang and Wei Wei and Ting-ting Yuan and Kui Zheng and Xiaoming Cui and Jie Li and Guangqian Pei and Xin Qiang and William Yiu-Man Cheung and Lianfeng Li and Fang-Fang Sun and Si Qin and Jicheng Huang and G. Leung and Edward C. Holmes and Yan-Ling Hu and Yi Guan and Wu-Chun Cao},
  journal={Nature},
  year={2020},
  volume={583},
  pages={282-285}
}
The ongoing outbreak of viral pneumonia in China and across the world is associated with a new coronavirus, SARS-CoV-2 1 . This outbreak has been tentatively associated with a seafood market in Wuhan, China, where the sale of wild animals may be the source of zoonotic infection 2 . Although bats are probable reservoir hosts for SARS-CoV-2, the identity of any intermediate host that may have facilitated transfer to humans is unknown. Here we report the identification of SARS-CoV-2-related… 

Isolation of SARS-CoV-2-related coronavirus from Malayan pangolins

It is shown that a coronavirus isolated from a Malayan pangolin has 100%, 98.6%, 97.8% and 90.7% amino acid identity with SARS-CoV-2 in the E, M, N and S proteins, respectively, which suggests that the latter may have originated from a recombination event involving Sars-related coronaviruses from bats and pangolins.

A novel SARS-CoV-2 related coronavirus in bats from Cambodia

The identification of SARS-CoV-2 related coronaviruses in two Rhinolophus shameli bats sampled in Cambodia in 2010 is described and suggests that Southeast Asia represents a key area to consider in the ongoing search for the origins of Sars-Cov-2, and in future surveillance for coronavIRuses.

Evidence for SARS-CoV-2 related coronaviruses circulating in bats and pangolins in Southeast Asia

Molecular and serological evidence of SARS-CoV-2 related coronaviruses (SC2r-CoVs) actively circulating in bats in Southeast Asia are reported and the geographic distribution of genetically diverse SC2r -CoVs from Japan and China to Thailand over a 4800-km range is extended.

Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor

RaTG15 receptor binding domain (RBD) can bind to and use Rhinolophus affinis bat ACE2 but not human ACE2 as entry receptor, although which contains a short deletion and has different key residues responsible for ACE2 binding.

SARS-CoV-2: Zoonotic origin of pandemic coronavirus.

The analysis of the SARS-CoV-2 genome shows that natural evolutionary process between a bat- coV and a pangolin- CoV or other animal coronavirus could have been important in creating SARS, with transmission of novel virus to the human population.

Functional comparison of SARS-CoV-2 with closely related pangolin and bat coronaviruses

The infectivity and antigenicity patterns of SARS-CoV-2 and the three related coronaviruses are studied and it is found that the latter can infect porcine cells, which could be partially attributed to an amino acid difference at the position of 498 of the spike protein.

Molecular evolution and phylogenetic analysis of SARS-CoV-2 and hosts ACE2 protein suggest Malayan pangolin as intermediary host

An evolutionary analysis combining viral and host phylogenies and divergence of ACE2 and TMPRSS2 amino acid sequences between CoV hosts finds pangolin has become an opportune host to intermediates bat-to-human SARS-CoV-2 jump and entry.

Genome evolution of SARS-CoV-2 and its virological characteristics

The phylogeny of SARS-CoV-2 is described, covering various related studies, in particular, focusing on viruses obtained from horseshoe bats and pangolins that belong to Sarbecovirus, a subgenus of Betacoronavirus.

A novel SARS-CoV-2 related coronavirus with complex recombination isolated from bats in Yunnan province, China

The discovery of PrC31 supports that bats are the natural hosts of SARS-CoV-2, and reveals that the beta-CoVs may possess more complicated recombination mechanism.
...

References

SHOWING 1-10 OF 49 REFERENCES

Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor

These results provide the strongest evidence to date that Chinese horseshoe bats are natural reservoirs of SARS-CoV, and that intermediate hosts may not be necessary for direct human infection by some bat SL-CoVs, and highlight the importance of pathogen-discovery programs targeting high-risk wildlife groups in emerging disease hotspots.

Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus

It is hypothesized that the direct progenitor of SARS-CoV may have originated after sequential recombination events between the precursors of these SARSr-CoVs, and highlights the necessity of preparedness for future emergence of Sars-like diseases.

Genomic characterization and infectivity of a novel SARS-like coronavirus in Chinese bats

Using pathogenic tests, it is found that the SARS-CoV virus can reproduce and cause disease in suckling rats, and further studies showed that the virus-like particles can be observed in the brains of sucking rats by electron microscopy.

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.

Identification of Diverse Alphacoronaviruses and Genomic Characterization of a Novel Severe Acute Respiratory Syndrome-Like Coronavirus from Bats in China

Diverse alphacoronaviruses and a close relative (LYRa11) to SARS CoV are identified in bats collected in Yunnan, China, indicating that most reported bat SARS-like CoVs are not the progenitors of human Sars CoV.

Bats Are Natural Reservoirs of SARS-Like Coronaviruses

It is reported that species of bats are a natural host of coronaviruses closely related to those responsible for the SARS outbreak, and these viruses display greater genetic variation than SARS-CoV isolated from humans or from civets.

Identification of Diverse Bat Alphacoronaviruses and Betacoronaviruses in China Provides New Insights Into the Evolution and Origin of Coronavirus-Related Diseases

Five SARS-related CoVs (SARSr-CoVs) in Rhinolophus bats from Sichuan and Yunnan are identified and it is confirmed that angiotensin-converting enzyme 2 usable SARSr -CoVs were continuously circulating in Rhine spp.

Receptor recognition by novel coronavirus from Wuhan: 2 An analysis based on decade-long structural studies of SARS 3

The potential receptor usage by 2019-nCoV is analyzed, based on the rich knowledge about SARS-CoV and the newly released sequence of 2019- nCoV, to provide insights into the receptor usage, cell entry, host cell infectivity and animal origin of the novel coronavirus.

Prevalence and Genetic Diversity of Coronaviruses in Bats from China

The findings of this study suggest that bats may play an integral role in the ecology and evolution of coronavirus ecology, in particular the role of bats and other wild animals.

Receptor Recognition by the Novel Coronavirus from Wuhan: an Analysis Based on Decade-Long Structural Studies of SARS Coronavirus

These analyses provide insights into the receptor usage, cell entry, host cell infectivity and animal origin of 2019-nCoV and may help epidemic surveillance and preventive measures against 2019- nCoV.