Identifying DNA sequences recognized by a transcription factor using a bacterial one-hybrid system

@article{Meng2006IdentifyingDS,
  title={Identifying DNA sequences recognized by a transcription factor using a bacterial one-hybrid system},
  author={Xiangdong Meng and Scot A. Wolfe},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={30-45}
}
Bacterial-based interaction trap systems provide a powerful method to identify interacting macromolecules. When carried out in the context of a genetic selection, interacting pairs can be rapidly isolated from large combinatorial libraries. This technology has been adapted to allow the identification of DNA-binding sequences for a transcription factor (TF) from a large randomized library. This procedure uses a library of randomized binding sites upstream of a cocistronic HIS3-URA3 reporter… CONTINUE READING

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