Identification of vitellogenin in the little skate (Raja erinacea).

  title={Identification of vitellogenin in the little skate (Raja erinacea).},
  author={L. E. Perez and Ian P. Callard},
  journal={Comparative biochemistry and physiology. B, Comparative biochemistry},
  volume={103 3},
1. Vitellogenin was isolated from mature female skates by selective precipitation with MgCl2/EDTA followed by chromatography on DEAE-cellulose columns. 2. A single monomer of approximately 205 kDa was identified on 6.0% SDS-PAGE gels. 3. In addition, isolation of yolk proteins with ammonium sulfate yielded proteins of 94 and 38 kDa (putative phosvitins) and putative lipovitellins of ca 105, 91 and 67 kDa. 4. In vivo phosphate incorporation in female and male skates implanted with estradiol… CONTINUE READING

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