Identification of valid reference genes during the differentiation of human myoblasts

  title={Identification of valid reference genes during the differentiation of human myoblasts},
  author={Jens stern-strAeter and Gabriel Alejandro Bonaterra and Karl Hoermann and Ralf Kinscherf and Urlich Reinhart Goessler},
  journal={BMC Molecular Biology},
  pages={66 - 66}
Analysis of RNA expression using real-time PCR (qRT-PCR) traditionally includes reference genes (RG) as an internal control. This practice is being questioned as it becomes increasingly clear that RG may vary considerably under certain experimental conditions. Thus, the validity of a particular RG must be determined for each experimental setting. We used qRT-PCR to measure the levels of six RG, which have been reported in the literature to be invariant. The RG were analyzed in human myoblast… CONTINUE READING


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