Identification of types of membrane injuries and cell death using whole cell-based proton-sensitive field-effect transistor systems.

@article{Imaizumi2017IdentificationOT,
  title={Identification of types of membrane injuries and cell death using whole cell-based proton-sensitive field-effect transistor systems.},
  author={Yukiko Imaizumi and Tatsuro Goda and Akira Matsumoto and Yuji Miyahara},
  journal={The Analyst},
  year={2017},
  volume={142 18},
  pages={
          3451-3458
        }
}
An NH4Cl-superfused system for a cell-cultured pH-sensing transistor was developed for detecting ion leakage across the plasma membranes of model HepG2 cells. The screening of chemical species by the method developed and conventional membrane-leakage assays identified the types of membrane injuries: structural membrane disruption and pore formation. Apoptosis-mediated membrane disordering was detected by continuously monitoring the ion-barrier breakdown of the membranes using the transistor… 
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