Identification of two related pentapeptides from the brain with potent opiate agonist activity
@article{Hughes1975IdentificationOT,
title={Identification of two related pentapeptides from the brain with potent opiate agonist activity},
author={John Hughes and T. W. Smith and Hans Walter Kosterlitz and Linda A. Fothergill and Barry A. Morgan and Howard R. Morris},
journal={Nature},
year={1975},
volume={258},
pages={577-579}
}Enkephalin, a natural ligand for opiate receptors is composed of the pentapeptides H–Tyr–Gly–Gly–Phe–Met–OH and H–Tyr–Gly–Gly–Phe–Leu–OH. The evidence is based on the determination of the amino acid sequence of natural enkephalin by the dansyl–Edman procedure and by mass spectrometry followed by synthesis and comparison of the natural and synthetic peptides.
3,544 Citations
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Hughes et al.5 have shown that the opiate activity isolated by them is due to a mixture of two peptides H–Tyr–Gly– Gly–Phe–Met–OH and H-Tyr-Gly-Phe-Leu–OH termed methionine-enkephalin and leucine- enkephaline respectively.
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