Identification of two novel esterases from a marine metagenomic library derived from South China Sea

@article{Chu2008IdentificationOT,
  title={Identification of two novel esterases from a marine metagenomic library derived from South China Sea},
  author={Xinmin Chu and Haoze He and C. Guo and Baolin Sun},
  journal={Applied Microbiology and Biotechnology},
  year={2008},
  volume={80},
  pages={615-625}
}
The demand for novel biocatalysts is increasing in modern biotechnology, which greatly stimulates the development of powerful tools to explore the genetic resources in the environment. Metagenomics, a culture independent strategy, provides an access to valuable genetic resources of the uncultured microbes. In this study, two novel esterase genes designated as estA and estB, which encoded 277- and 328-amino-acid peptides, respectively, were isolated from a marine microbial metagenomic library by… 

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References

SHOWING 1-10 OF 35 REFERENCES
Screening for novel lipolytic enzymes from uncultured soil microorganisms
TLDR
The construction of a metagenomic library in Escherichia coli using fosmid and microbial DNA directly isolated from forest topsoil and screened for lipolytic enzymes is presented and six predicted gene products were successfully expressed in E. coli and secreted into the culture broth.
Isolation and Biochemical Characterization of Two Novel Metagenome-Derived Esterases
TLDR
The metagenomes of uncultured microbial communities are rich sources for novel biocatalysts and esterase EstA3 and EstCE1 were derived from a drinking water and soil metagenome, respectively, and display remarkable characteristics that cannot be related to the original environment.
Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment.
TLDR
A novel lipase was isolated from a metagenomic library of Baltic Sea sediment bacteria and Conserved regions, including the putative active site, GDSAG, a catalytic triad (Ser148, Glu242 and His272) and a HGG motif, were identified.
Isolation of novel lipolytic genes from uncultured bacteria of pond water.
Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin.
Construction, Analysis, and β-Glucanase Screening of a Bacterial Artificial Chromosome Library from the Large-Bowel Microbiota of Mice
TLDR
A metagenomic (community genomic) library consisting of 5,760 bacterial artificial chromosome clones was prepared in Escherichia coli DH10B from DNA extracted from the large-bowel microbiota of BALB/c mice, and putative genes were detected, some of which might have a role in environmental sensing, nutrient acquisition, or coaggregation.
Expression and isolation of antimicrobial small molecules from soil DNA libraries.
TLDR
Results show that genes involved in natural product synthesis can be cloned directly from S-DNA and expressed in a heterologous host, supporting the idea that this technology has the potential to provide novel natural products from the wealth of environmental microbial diversity and is a potentially important new tool for drug discovery.
Overexpression and properties of a new thermophilic and thermostable esterase from Bacillus acidocaldarius with sequence similarity to hormone-sensitive lipase subfamily.
TLDR
The enzyme, a 34 kDa monomeric protein, was demonstrated to be a B'-type carboxylesterase (EC 3.1.1) on the basis of substrate specificity and the action of inhibitors, and supported the previous suggestion of a catalytic triad made up of Ser-His-Asp.
Novel natural products from soil DNA libraries in a streptomycete host.
As a route to accessing the potential chemical diversity of uncultivable microbes from the soil, combinatorial biosynthetic libraries were constructed by cloning large fragments of DNA isolated from
...
1
2
3
4
...