Identification of two neutrophil chemotactic peptides produced by porcine alveolar macrophages.

@article{Goodman1991IdentificationOT,
  title={Identification of two neutrophil chemotactic peptides produced by porcine alveolar macrophages.},
  author={Richard C. Goodman and John W. Forstrom and Sherri G. Osborn and Emil Y. Chi and Thomas R. Martin},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 13},
  pages={8455-63}
}
We have purified to homogeneity two distinct 10-kDa proteins with potent chemotactic activity for neutrophils from porcine alveolar macrophages incubated for 24 h with Escherichia coli endotoxin (lipopolysaccharide (LPS), 10 micrograms/ml). Neutrophil chemotactic activity in alveolar macrophage supernatants was concentrated by adsorption to SP-Sephadex, and purified by cation exchange and reversed phase high performance liquid chromatography. The first peptide, alveolar macrophage chemotactic… CONTINUE READING