Identification of twenty novel polymorphic microsatellite DNA markers from transcripts of the pearl oyster Pinctada fucata using next-generation sequencing approach

@article{Fan2014IdentificationOT,
  title={Identification of twenty novel polymorphic microsatellite DNA markers from transcripts of the pearl oyster Pinctada fucata using next-generation sequencing approach},
  author={Sigang Fan and Jingxuan Wang and Gui-ju Huang and Bao-suo Liu and Dahui Yu},
  journal={Journal of Genetics},
  year={2014},
  volume={94},
  pages={82-85}
}
The pearl oyster, Pinctada fucata (Gould 1850) is a commercially important marine shellfish cultured for producing saltwater pearls mainly in China and Japan (Yu and Chu 2006). It is common in most areas of tropical and subtropical oceans and seas in the Pacific and Indian regions. In 1965, this specie was successfully propagated and reared under artificial conditions in Guangxi province in southern China and expanded rapidly to the neighbouring Guangdong and Hainan provinces subsequently (Meng… 
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References

SHOWING 1-10 OF 29 REFERENCES
Isolation and screening of microsatellite markers from the Chinese pearl oyster Pinctada fucata based on FIASCO
TLDR
It is demonstrated that FIASCO is a useful technique of microsatellite isolation for Pinctada fucata and nine loci showed polymorphism as tested by a population.
Development of EST-derived microsatellite markers in the pearl oyster Pinctada martensii (Dunker) for genetic resource monitoring
TLDR
Results revealed significant genetic differentiation between the wild and hatchery populations of Pinctada martensii and most of those loci are in Hardy–Weinberg equilibrium.
Development, characterization, and inheritance of 113 novel EST-SSR markers in the Pacific oyster (Crassostrea gigas)
TLDR
A total of 113 novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the Pacific oyster (Crassotrea gigas) and will help in investigation of QTL mapping and genetic diversity in this species.
Rapid isolation and characterization of microsatellites from the genome of pearl oyster (Pinctada martensi Dunker)
TLDR
A new set of 34 microsatellite markers was isolated and characterized in pearl oyster Pincada martensi from a CA16-enriched genomic library and showed high genetic variability represented mainly by the number of alleles and polymorphic information content.
Development of 25 Novel Microsatellite Loci and Genetic Variation Analysis in Breeding Populations of the Pearl Oyster, Pinctada fucata
In this study, we screened 103,050 gene sequences of Pinctada fucata and identified 1450 simple sequence repeats (SSRs) distributing in 1355 sequences, in which 97 have sufficient flanking sequences
Draft Genome of the Pearl Oyster Pinctada fucata: A Platform for Understanding Bivalve Biology
TLDR
The draft genome of the pearl oyster provides a platform for the identification of selection markers and genes for calcification, knowledge of which will be important in the pearl industry.
Development and characterization of six new microsatellite markers for the silver-or gold-lipped pearl oyster, Pinctada maxima (Pteriidae)
TLDR
Six di-, tri- and tetranucleotide microsatellite loci were developed for the silver- or gold-lipped pearl oyster Pinctada maxima using a linker-ligated, magnetic bead enrichment protocol and should be very useful in DNA parentage analyses and population differentiation of P. maxima in Australia and Indonesia.
Expression Profiling without Genome Sequence Information in a Non-Model Species, Pandalid Shrimp (Pandalus latirostris), by Next-Generation Sequencing
TLDR
The present study showed a de novo assembly approach for the transcriptome of a non-model species using only short-read sequence data, and provides a strategy for identifying sequences showing significantly different expression levels between populations.
Screening of microsatellite markers from Pinctada fucata based on a family population
TLDR
In this study, 35 out of 170 pairs of primers designed by the lab which can be effectively amplified, and 30 loci showed polymorphism as tested on a F2 population of P.fucata are suggested to be new markers for genetic diversity analysis, genetic mapping and molecular marker assisted selection for Pinctada fucata.
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