Identification of twenty novel polymorphic microsatellite DNA markers from transcripts of the pearl oyster Pinctada fucata using next-generation sequencing approach

  title={Identification of twenty novel polymorphic microsatellite DNA markers from transcripts of the pearl oyster Pinctada fucata using next-generation sequencing approach},
  author={Sigang Fan and Jingxuan Wang and Gui-ju Huang and Bao-suo Liu and Dahui Yu},
  journal={Journal of Genetics},
The pearl oyster, Pinctada fucata (Gould 1850) is a commercially important marine shellfish cultured for producing saltwater pearls mainly in China and Japan (Yu and Chu 2006). It is common in most areas of tropical and subtropical oceans and seas in the Pacific and Indian regions. In 1965, this specie was successfully propagated and reared under artificial conditions in Guangxi province in southern China and expanded rapidly to the neighbouring Guangdong and Hainan provinces subsequently (Meng… 
Identification of 15 novel polymorphic microsatellite loci in pearl oyster (Pinctada fucata).
The results suggest that the markers isolated in this study can be used for research on molecular marker-assisted selection and genetic variance of P. fucata.
Development of polymorphic microsatellites for genetic studies of white scar oyster (Crassostrea belcheri) using paired-end shotgun sequencing.
Genome information and 20 newly isolated microsatellites will facilitate further studies in population genetics, stock management, and genetic improvement of C. belcheri in Thailand.
Characterization of novel genotyping-by-sequencing (GBS)-based simple sequence repeats (SSRs) and their application for population genomics of Capoeta aculeata (Valenciennes, 1844).
Next generation sequencing technology is implemented to identify polymorphic microsatellite markers and investigate the population genetic structure of C. aculeata sampled from three geographical sites in Iran, finding these micros satellite markers could provide a valuable tool for future population and conservation genetics studies of this species.
De novo assembly of genome and development of polymorphic microsatellite loci in the blue swimming crab (Portunus pelagicus) using RAD approach
This study provided massive genetic resource and polymorphic molecular markers that should be helpful for studies on conservation genetics, population dynamics and genetic diversity of P. pelagicus and related crab species.
Journal of Genetics Online Resources
Identification of forty-five gene-derived polymorphic microsatellite loci for the sea cucumber, Apostichopus japonicus. Evaluation of genetic diversity of Portuguese Pinus sylvestris L. populations


Isolation and screening of microsatellite markers from the Chinese pearl oyster Pinctada fucata based on FIASCO
It is demonstrated that FIASCO is a useful technique of microsatellite isolation for Pinctada fucata and nine loci showed polymorphism as tested by a population.
Development of EST-derived microsatellite markers in the pearl oyster Pinctada martensii (Dunker) for genetic resource monitoring
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Rapid isolation and characterization of microsatellites from the genome of pearl oyster (Pinctada martensi Dunker)
A new set of 34 microsatellite markers was isolated and characterized in pearl oyster Pincada martensi from a CA16-enriched genomic library and showed high genetic variability represented mainly by the number of alleles and polymorphic information content.
Development of 25 Novel Microsatellite Loci and Genetic Variation Analysis in Breeding Populations of the Pearl Oyster, Pinctada fucata
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Draft Genome of the Pearl Oyster Pinctada fucata: A Platform for Understanding Bivalve Biology
The draft genome of the pearl oyster provides a platform for the identification of selection markers and genes for calcification, knowledge of which will be important in the pearl industry.
Development and characterization of six new microsatellite markers for the silver-or gold-lipped pearl oyster, Pinctada maxima (Pteriidae)
Six di-, tri- and tetranucleotide microsatellite loci were developed for the silver- or gold-lipped pearl oyster Pinctada maxima using a linker-ligated, magnetic bead enrichment protocol and should be very useful in DNA parentage analyses and population differentiation of P. maxima in Australia and Indonesia.
Expression Profiling without Genome Sequence Information in a Non-Model Species, Pandalid Shrimp (Pandalus latirostris), by Next-Generation Sequencing
The present study showed a de novo assembly approach for the transcriptome of a non-model species using only short-read sequence data, and provides a strategy for identifying sequences showing significantly different expression levels between populations.
Screening of microsatellite markers from Pinctada fucata based on a family population
In this study, 35 out of 170 pairs of primers designed by the lab which can be effectively amplified, and 30 loci showed polymorphism as tested on a F2 population of P.fucata are suggested to be new markers for genetic diversity analysis, genetic mapping and molecular marker assisted selection for Pinctada fucata.