Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer


BACKGROUND Tumour cells and stromal cells interact in the tumour microenvironment; moreover, stromal cells can acquire abnormalities that contribute to tumour progression. However, interactions between lymphatic endothelial cells (LECs) and tumour cells are largely unexamined. In this study, we aimed to determine whether tumour-specific LECs inhabit the tumour microenvironment and examine their influence on this microenvironment. METHODS We isolated normal LECs (NLECs) from a non-metastatic lymph node and tumour-associated LECs (TLECs) from cancerous lymph nodes. We examined proliferative and migratory potency, growth factor production, and gene expression of each type of LEC. Moreover, we developed a co-culture system to investigate the interactions between gastric cancer cells and LECs. RESULTS When compared with NLEC, TLECs had an abnormal shape, high proliferative and migratory abilities, and elevated expression of genes associated with inflammation, cell growth, and cell migration. NLECs co-cultured with gastric cancer cells from the OCUM12 cell line acquired TLEC-like phenotypes. Also, OCUM12 cells co-cultured with TLECs expressed high levels of genes responsible for metastasis. CONCLUSIONS Our results demonstrated that LECs interacted with tumour cells and obtained abnormal phenotypes that could have important roles in tumour progression.

DOI: 10.1038/bjc.2015.282

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@inproceedings{Tokumoto2015IdentificationOT, title={Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer}, author={Mao Tokumoto and Hiroaki Tanaka and Yukie Tauchi and Hiroaki Kasashima and Kento Kurata and Masakazu Yashiro and Katsunobu Sakurai and Takahiro Toyokawa and Naoshi Kubo and Ryosuke Amano and Kenjiro Kimura and Kazuya Muguruma and Kiyoshi Maeda and Masaichi Ohira and Kosei Hirakawa}, booktitle={British journal of cancer}, year={2015} }