Identification of transient glycosylation alterations of sialylated mucin oligosaccharides during infection by the rat intestinal parasite Nippostrongylus brasiliensis.

@article{Karlsson2000IdentificationOT,
  title={Identification of transient glycosylation alterations of sialylated mucin oligosaccharides during infection by the rat intestinal parasite Nippostrongylus brasiliensis.},
  author={N. Karlsson and F. Olson and P. Jovall and Y. Andersch and L. Enerb{\"a}ck and G. Hansson},
  journal={The Biochemical journal},
  year={2000},
  volume={350 Pt 3},
  pages={
          805-14
        }
}
The sialylation of the oligosaccharides from small-intestinal mucins during a 13-day infectious cycle was studied in Sprague-Dawley rats with the parasite Nippostrongylus brasiliensis. Sialic acid analysis and release, permethylation and analysis by GC-MS of the sialylated oligosaccharides isolated from the 'insoluble' mucin complex revealed a relative decrease (4-7-fold) of N-glycolylneuraminic acid compared with N-acetylneuraminic acid just before parasite expulsion. Northern blots showed… Expand
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