Identification of toxS, a regulatory gene whose product enhances toxR-mediated activation of the cholera toxin promoter.

@article{Miller1989IdentificationOT,
  title={Identification of toxS, a regulatory gene whose product enhances toxR-mediated activation of the cholera toxin promoter.},
  author={Virginia L. Miller and Victor J DiRita and John J Mekalanos},
  journal={Journal of bacteriology},
  year={1989},
  volume={171 3},
  pages={1288-93}
}
We describe the cloning of the toxS gene from Vibrio cholerae E1 Tor strain E7946. This gene lies downstream from the toxR gene, which encodes the transcriptional activator for the cholera toxin (ctx) operon in V. cholerae. We show that ToxS acts in conjunction with ToxR to activate expression of the ctx operon in Escherichia coli. The classical strain 569B, which is attenuated for virulance but which synthesizes high levels of cholera toxin in vitro, carries a deletion of 1.2 kilobase pairs of… CONTINUE READING