Identification of three novel salicylate 1-hydroxylases involved in the phenanthrene degradation of Sphingobium sp. strain P2.

@article{Pinyakong2003IdentificationOT,
  title={Identification of three novel salicylate 1-hydroxylases involved in the phenanthrene degradation of Sphingobium sp. strain P2.},
  author={Onruthai Pinyakong and Hiroshi Habe and Takako Yoshida and Hideaki Nojiri and Toshio Omori},
  journal={Biochemical and biophysical research communications},
  year={2003},
  volume={301 2},
  pages={350-7}
}
Five sets of large and small subunits of terminal oxygenase (ahdA1[a-e] and ahdA2[a-e]) and a single gene set encoding ferredoxin (ahdA3) and ferredoxin reductase (ahdA4) were found to be scattered through 15.8- and 14-kb DNA fragments of phenanthrene-degrading Sphingobium sp. strain P2. RT-PCR analysis indicated the inducible and specific expression of ahdA3, ahdA4, and three sets of genes for terminal oxygenase (ahdA1[c-e] and ahdA2[c-e]) in this strain grown on phenanthrene. The… CONTINUE READING

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