• DOI:10.1007/BF00431588
• Corpus ID: 9751945

Identification of the tRNA-binding sites on rat liver ribosomes by affinity labeling

@article{Czernilofsky2004IdentificationOT,
  title={Identification of the tRNA-binding sites on rat liver ribosomes by affinity labeling},
  author={A. P. Czernilofsky and Ekkehard Collatz and Axel M. Gressner and Ira G. Wool and Ernst K{\"u}chler},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={153},
  pages={231-235}
}

• A. Czernilofsky, E. Collatz, +2 authors E. Küchler
• Published 2004
• Biology
• Molecular and General Genetics MGG

Summaryp-Nitrophenoxycarbonyl-3H-phenylalanyl-tRNAyeastPhebinds to 80S rat liver ribosomes and forms a covalent bond with ribosomal proteins. The affinity labeled proteins were identified by a kind of “three-dimensional” electrophoresis: groups of 80S proteins were cut out of two-dimensional gel slabs, eluted, and separated by electrophoresis in polyacrylamide gels containing sodium dodecyl sulfate; finally, the radioactivity in the protein bands was determined. The proteins L32/L33, L36, L21, L23, L28/L29 and L13 were labeled when the reaction was with poly(U) present. We conclude they are located at or near the peptidyl transferase center. Proteins S7 and/or L9 and a protein in the vicinity of L36 which could not be identified were more radioactive when the affinity reaction was in the absence of poly(U).