Identification of the subunits of F1F0-ATPase from bovine heart mitochondria.

  title={Identification of the subunits of F1F0-ATPase from bovine heart mitochondria.},
  author={John E Walker and Ren{\'e} Lutter and Alain Dupuis and Michael J. Runswick},
  volume={30 22},
An oligomycin-sensitive F1F0-ATPase isolated from bovine heart mitochondria has been reconstituted into phospholipid vesicles and pumps protons. this preparation of F1F0-ATPase contains 14 different polypeptides that are resolved by polyacrylamide gel electrophoresis under denaturing conditions, and so it is more complex than bacterial and chloroplast enzymes, which have eight or nine different subunits. The 14 bovine subunits have been characterized by protein sequence analysis. They have been… CONTINUE READING


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