Identification of the sex of a wide range of Carinatae birds by PCR using primer sets selected from chicken EE0.6 and its related sequences.

@article{Itoh2001IdentificationOT,
  title={Identification of the sex of a wide range of Carinatae birds by PCR using primer sets selected from chicken EE0.6 and its related sequences.},
  author={Yuichi Itoh and Miho Suzuki and Akira Ogawa and Isao Munechika and Koichi Murata and Satoshi Mizuno},
  journal={The Journal of heredity},
  year={2001},
  volume={92 4},
  pages={315-21}
}
A 0.6 kb EcoRI fragment (EE0.6), cloned from the W chromosome of chickens, is a nonrepetitive sequence and contains an exonlike sequence, ET15, which is likely a part of a pseudogene. The EE0.6 sequence is conserved in all species of birds examined both in Carinatae and Ratitae. A counterpart sequence of EE0.6 is present on the Z chromosome. The extent of diversity between the W- and Z-linked sequences are variable among species. The W- and Z-linked EE0.6 sequences, cloned from 12 different… CONTINUE READING