Identification of the series-specific random amplified polymorphic DNA markers of Viola species

  title={Identification of the series-specific random amplified polymorphic DNA markers of Viola species},
  author={B. Oh and M. Ko and C. Lee},
  journal={Plant Breeding},
Although many Viola species are grown as ornamentals, no information on molecular markers is available for the classification and breeding of Viola. Random amplified polymorphic DNA (RAPD) analysis was applied to identify series-specific molecular markers from the series Pinnatae, Chinensis, and Variegatae of Viola species in the subsection Patellares. We report eight RAPD markers which can be used to distinguish three different series of Viola species based on morphological characters. The… Expand
Evaluation of Genetic Diversity in Iranian Violet (Viola spp) Populations Using Morphological and RAPD Molecular Markers
The results of the molecular and morphological analysis showed considerable diversity among violet populations in Iran, which can be used in future breeding programs. Expand
Characterisation of genetic relationships in pansy (Viola wittrockiana) inbred lines using morphological traits and RAPD markers
Summary In this study, genetic relationships between eighteen inbred lines of pansy (Viola wittrockiana) were estimated on the basis of morphological characters and RAPD data. Thirty-oneExpand
Morphological versus genetic diversity of Viola reichenbachiana and V. riviniana (sect. Viola, Violaceae) from soils differing in heavy metal content.
It is concluded that plants forming the M populations are well adapted to a metal-polluted environment, and could be considered as stabilised introgressive forms resulting from unidirectional (asymmetric) introgression toward V. riviniana. Expand
Allozyme diversity in natural populations of Viola palmensis Webb & Berth. (Violaceae) from La Palma (Canary Islands): implications for conservation genetics.
For conservation genetics, in situ preservation areas are defined that are free of disturbance and that include populations with the highest genetic diversity, suggesting the threatened Canarian endemic Viola palmensis Webb & Berth might contribute to pollen dispersion of this species. Expand
Hybridization between insular endemic and widespread species of Viola in non-disturbed environments assessed by nuclear ribosomal and cpDNA sequences
The additivity of the ITS sequences, together with the pollen and ovule sterility, suggests that the sampled individuals are primary F1 hybrids, whereas no trace of introgressive hybridization or hybrid zone has been evidenced by the nuclear and plastid markers used. Expand


DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA. Expand
Fingerprinting genomes using PCR with arbitrary primers.
The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa. Expand