Identification of the second chromophore of Escherichia coli and yeast DNA photolyases as 5,10-methenyltetrahydrofolate.

@article{Johnson1988IdentificationOT,
  title={Identification of the second chromophore of Escherichia coli and yeast DNA photolyases as 5,10-methenyltetrahydrofolate.},
  author={Jean Luc Johnson and Sarah F Hamm-Alvarez and G P Payne and Gwendolyn B. Sancar and Krishnakumar Rajagopalan and Aziz Sancar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1988},
  volume={85 7},
  pages={2046-50}
}
Denaturation of DNA photolyase (deoxyribodipyrimidine photolyase, EC 4.1.99.3) from Escherichia coli with guanidine hydrochloride or acidification to pH 2 released, in addition to FAD, a chromophore with the spectral and chromatographic properties of a reduced pterin. Treatment of the enzyme with iodine prior to acidification converted the chromophore to a stable, oxidized derivative, which was resolved by HPLC into four species with identical spectral properties. The same species, in the same… CONTINUE READING

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