Identification of the remains of the Romanov family by DNA analysis

  title={Identification of the remains of the Romanov family by DNA analysis},
  author={Peter Gill and Pavel L. Ivanov and Colin P. Kimpton and Romelle Piercy and Nicola Benson and Gillian Tully and Ian W. Evett and Erika Hagelberg and Kevin M. Sullivan},
  journal={Nature Genetics},
Nine skeletons found in a shallow grave in Ekaterinburg, Russia, in July 1991, were tentatively identified by Russian forensic authorities as the remains of the last Tsar, Tsarina, three of their five children, the Royal Physician and three servants. We have performed DNA based sex testing and short tandem repeat (STR) analysis and confirm that a family group was present in the grave. Analysis of mitochondrial (mt) DNA reveals an exact sequence match between the putative Tsarina and the three… 
Forensic identification of skeletal remains from members of Ernesto Che Guevara’s guerrillas in Bolivia based on DNA typing
The positive identification of several members of the guerrillas led by Ernesto “Che” Guevara on the 1960 s in Bolivia by means of DNA fingerprinting is reported.
The execution of the Romanov family at Yekatarinberg
  • R. Byard
  • History
    Forensic Science, Medicine and Pathology
  • 2020
There appears little doubt that the skeletal remains in the two graves outside Yekaterinburg are those of Tsar Nicholas, his wife and their five children, and the genetic analyses and the features of the fragmented remains are all very consistent with the tragic story of the last days of the Romanov family and with the subsequent desecration and destruction of their bodies.
Mitochondrial DNA sequence heteroplasmy in the Grand Duke of Russia Georgij Romanov establishes the authenticity of the remains of Tsar Nicholas II
The skeletal remains of the Tsar's brother Georgij Romanov were analysed, at the request of the Russian Federation government, in order to gain further insight into the occurrence and segregation of heteroplasmic mtDNA variants in theTsar's maternal lineage.
Identification of remains by sequencing of mitochondrial DNA control region.
The maternity of two newborns who were murdered and abandoned >5 and 10 years were analyzed by amplification and direct sequencing of mitochondrial DNA control regions, demonstrating that sequencing of mtDNA is a useful tool for genetic identification of aged and decomposed materials.
Ancient DNA and Family Relationships in a Pompeian House
Six of the thirteen skeletons found in a house at the Pompeii archaeological site, dated to 79 A.D, are indeed closely related, and their relationships are identified.
Mitochondrial DNA analysis of the presumptive remains of Jesse James.
The mtDNA analysis supports the identification of the exhumed remains from Mt. Olivet Cemetery as those of Jesse James, and searches the forensic mtDNA database found that this sequence does not appear among the 2426 mtDNA sequences therein.
An Analysis of the Alleged Skeletal Remains of Carin Göring
Nuclear DNA analysis of skeletal remains believed to belong to Carin Göring revealed one shared allele for each of the three markers, supporting a mother and son relationship, and genetic information together with anthropological and historical files provides an additional piece of circumstantial evidence.
Genetic analysis of the presumptive blood from Louis XVI, King of France.


Identification of the skeletal remains of a murder victim by DNA analysis
This analysis establishes the authenticity of the bone DNA and the feasibility of bone DNA typing in forensic investigations, and reports the successful identification of the 8-year-old skeletal remains of a murder victim by comparative typing of nuclear microsatellite markers3.
Mitochondrial DNA sequences from a 7000-year old brain.
The sequences show that this ancient individual belonged to a mitochondrial lineage that is rare in the Old World and not previously known to exist among Native Americans, bringing to three the number of maternal lineages known to have been involved in the prehistoric colonization of the New World.
Isolation and characterization of DNA from archaeological bone
  • E. Hagelberg, J. Clegg
  • Biology
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 1991
DNA was extracted from human and animal bones recovered from archaeological sites and mitochondrial DNA sequences were amplified from the extracts using the polymerase chain reaction to show that significant amounts of genetic information can survive for long periods in bone.
Replacement of bovine mitochondrial DNA by a sequence variant within one generation.
Thirteen instances were detected of mother-daughter pairs in which leukocytes of each of the two animals seemingly were homoplasmic for a different allele at nucleotide 364, demonstrating the bovine mitochondrial genome can be replaced completely by a nucleotide sequence variant within a single generation.
A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X-Y homologous gene amelogenin.
Quantitation enables sex chromosome aneuploidy to be determined, and the amelogenin intron sequence can also be co-amplified with several highly polymorphic microsatellite loci, thereby providing a combined gender/identity DNA test.
Automated amplification and sequencing of human mitochondrial DNA
A protocol which enables mitochondrial sequence information to be generated rapidly and automatically is described, likely to be of importance in forensic analysis where the DNA is too degraded or of insufficient quantity to be analysed by other techniques.
DNA typing with fluorescently tagged short tandem repeats: a sensitive and accurate approach to human identification.
PCR-based DNA typing with fluorescent STR primers and automated analysis provides the enhanced level of precision, accuracy and sensitivity required for forensic casework analysis.
New approaches to dating suggest a recent age for the human mtDNA ancestor.
Two approaches for deriving an intraspecific calibration of the rate of human mtDNA sequence evolution that allow standard errors to be readily calculated are presented.
Tetranucleotide repeat polymorphism at the human beta-actin related pseudogene 2 (ACTBP2) detected using the polymerase chain reaction.
The polymorphic sequence (TG)25 lies m intron IV, 61 bases 5' from the start of exon 5 of the human alpha-cardiac actin gene (1) was identified from a search of the EMBL and GenBank DNA sequence databases (GenBank J00072).