Identification of the region of rho involved in substrate recognition by Escherichia coli cytotoxic necrotizing factor 1 (CNF1).

@article{Lerm1999IdentificationOT,
  title={Identification of the region of rho involved in substrate recognition by Escherichia coli cytotoxic necrotizing factor 1 (CNF1).},
  author={Maria Lerm and Gudula Schmidt and U M Goehring and J{\"o}rg Schirmer and Klaus Aktories},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 41},
  pages={28999-9004}
}
The Escherichia coli cytotoxic necrotizing factor 1 (CNF1) and the Bordetella dermonecrotic toxin (DNT) activate Rho GTPases by deamidation of Gln(63) of RhoA (Gln(61) of Cdc42 and Rac). In addition, both toxins possess in vitro transglutaminase activity in the presence of primary amines. Here we characterized the region of Rho essential for substrate recognition by the toxins using Rho/Ras chimeras as protein substrates. The chimeric protein Ras55Rho was deamidated or transglutaminated by CNF1… CONTINUE READING