Identification of the polymerase polyprotein products p72 and p65 of the murine coronavirus MHV-JHM.

@article{Gao1996IdentificationOT,
  title={Identification of the polymerase polyprotein products p72 and p65 of the murine coronavirus MHV-JHM.},
  author={H Q Gao and Jennifer J Schiller and Susan C Baker},
  journal={Virus research},
  year={1996},
  volume={45 2},
  pages={101-9}
}
The RNA polymerase gene of murine coronavirus MHV-JHM encodes a polyprotein of greater than 750 kDa. This polyprotein is proposed to be processed by two papain-like cysteine proteinases, PCP-1 and PCP-2, and a poliovirus 3C-like proteinase domain, 3C-pro, to generate protein products. The amino-terminal product of the MHV polymerase polyprotein, p28, is generated by cleavage of the polyprotein by PCP-1. To identify the viral products downstream of p28, we generated a fusion-protein specific… CONTINUE READING