Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12.

@article{Yang1996IdentificationOT,
  title={Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12.},
  author={Yanan Yang and Guo-Ping Zhao and Malcolm E Winkler},
  journal={FEMS microbiology letters},
  year={1996},
  volume={141 1},
  pages={89-95}
}
We isolated a miniTn10(Cmr) insertion mutant lacking pyridoxine (PN) kinase and cloned the structural gene, designated pdxK, by complementation. P1 transduction and PCR mapping and DNA sequence analysis showed that pdxK was adjacent to the crr sugar transport gene (53.95 min). Growth properties of pdxK::miniTn10 mutants supported the hypotheses that PN kinase, which also phosphorylates pyridoxal (PL) and pyridoxamine (PM) in vitro, functions solely in the B6-vitamer salvage pathway and that E… CONTINUE READING

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