Identification of the oligosaccharide structures of human coagulation factor X activation peptide at each glycosylation site

Abstract

Human blood coagulation factor X has two N-linked oligosaccharides at Asn39 and Asn49 residues and two O-linked oligosaccharides at Thr17 and Thr29 residues in the region of the factorX activationpeptide (XAP) which is cleaved off during its activation by factor IXa. We determined the structure of oligosaccharides in the XAP region of human factor X. Four glycopeptides each containing a glycosylation site were isolated by digestion of XAP with endoproteinase Asp-N followed by reversed-phase HPLC. N-linked oligosaccharides released from the glycopeptides by glycoamidase A digestion were derivatized with 2-aminopyridine. Pyridylamino(PA)-oligosaccharides were separated by HPLC into neutral and sialyl oligosaccharides using an anion-exchange column. Structures of oligosaccharides and their contents at each glycosylation site were determined by a two-dimensional sugar mapping method. The contents of the neutral oligosaccharides at Asn39 and Asn49 residues were 32.5% and 30.0%, respectively. Six neutral and twelve monosialyl oligosaccharides isolated from both N-linked glycosylation sites showed similar elution profiles composed of bi-, tri-and tetra-antennary complex type oligosaccharides. The predominant component in neutral oligosaccharides was biantennary without a fucose residue. Two major monosialyl oligosaccharides were also biantennary without fucose and with a Neu5Acα-2→6 residue. In addition, the structures of O-linked oligosaccharides at Thr17 and Thr29 residues were suggested to be disialylated Gal/β3GalNAc sequences by their component analyses.

DOI: 10.1007/BF00731362

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Cite this paper

@article{Nakagawa1995IdentificationOT, title={Identification of the oligosaccharide structures of human coagulation factor X activation peptide at each glycosylation site}, author={Hiroaki Nakagawa and Noriko Takahashi and Kazuhisa Fujikawa and Yoshiya Kawamura and Masaki Iino and Hiroyuki Takeya and Hiroyuki Ogawa and Koji Suzuki}, journal={Glycoconjugate Journal}, year={1995}, volume={12}, pages={173-181} }