Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis.

@article{Moore2002IdentificationOT,
  title={Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis.},
  author={Jonathan D. Moore and Sally Kornbluth and Tim Hunt},
  journal={Molecular biology of the cell},
  year={2002},
  volume={13 12},
  pages={
          4388-400
        }
}
Cyclin-dependent kinase (Cdk)2/cyclin E is imported into nuclei assembled in Xenopus egg extracts by a pathway that requires importin-alpha and -beta. Here, we identify a basic nuclear localization sequence (NLS) in the N-terminus of Xenopus cyclin E. Mutation of the NLS eliminated nuclear accumulation of both cyclin E and Cdk2, and such versions of cyclin E were unable to trigger DNA replication. Addition of a heterologous NLS from SV40 large T antigen restored both nuclear targeting of Cdk2… CONTINUE READING

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