Identification of the new T-cell-stimulating antigens from Mycobacterium tuberculosis culture filtrate.

@article{Lim2004IdentificationOT,
  title={Identification of the new T-cell-stimulating antigens from Mycobacterium tuberculosis culture filtrate.},
  author={J H Lim and Hwa-Jung Kim and Kil-Soo Lee and Eun Kyeong Jo and Chang-Hwa Song and Saet-Byel Jung and Su-Young Kim and J C Lee and T W Paik and Jeong-kyu Park},
  journal={FEMS microbiology letters},
  year={2004},
  volume={232 1},
  pages={51-9}
}
The proteins secreted by Mycobacterium tuberculosis are an important target for vaccine development. To identify the antigens from M. tuberculosis culture filtrate (CF) that strongly stimulate T-cells, the CF was fractionated by ion-exchange chromatography and then non-reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis with mini-whole gel elution. Each fraction was screened for its ability to induce interferon-gamma (IFN-gamma) production in peripheral blood mononuclear cells… CONTINUE READING