Identification of the major membrane and core proteins of vaccinia virus by two-dimensional electrophoresis.

@article{Jensen1996IdentificationOT,
  title={Identification of the major membrane and core proteins of vaccinia virus by two-dimensional electrophoresis.},
  author={Ole N\orregaard Jensen and Tony Houthaeve and Andrej Shevchenko and Sally Cudmore and T Ashford and Melissa J. Mann and Gareth Griffiths and Jacomine Krijnse Locker},
  journal={Journal of virology},
  year={1996},
  volume={70 11},
  pages={7485-97}
}
Vaccinia virus assembly has been well studied at the ultrastructural level, but little is known about the molecular events that occur during that process. Towards this goal, we have identified the major membrane and core proteins of the intracellular mature virus (IMV). Pure IMV preparations were subjected to Nonidet P-40 (NP-40) and dithiothreitol (DTT) treatment to separate the core proteins from the membrane proteins. These proteins were subsequently separated by two-dimensional (2D) gel… CONTINUE READING

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