Identification of the major Ascaris allergen and its purification to homogeneity by high-performance liquid chromatography.

@article{McGibbon1990IdentificationOT,
  title={Identification of the major Ascaris allergen and its purification to homogeneity by high-performance liquid chromatography.},
  author={A M McGibbon and Jacinda Christie and Malcolm W Kennedy and Terry D. Lee},
  journal={Molecular and biochemical parasitology},
  year={1990},
  volume={39 2},
  pages={163-71}
}
The major allergen from the body fluid of adult Ascaris suum (ABF) has been identified and purified to homogeneity using gel permeation high-performance liquid chromatography (HPLC). The purity of the Ascaris body fluid allergen (ABA-1) was confirmed by HPLC and SDS-PAGE. ABA-1 appears to be a 25-kDa dimer in its native form, which runs as a 10-kDa monomer on SDS-PAGE under reducing conditions. The allergenicity of the HPLC-purified protein was confirmed using isolated in vivo-sensitised mast… CONTINUE READING

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