Identification of the human cytochromes p450 responsible for in vitro formation of R- and S-norfluoxetine.

@article{Ring2001IdentificationOT,
  title={Identification of the human cytochromes p450 responsible for in vitro formation of R- and S-norfluoxetine.},
  author={Barbara J. Ring and James A. Eckstein and Jennifer S. Gillespie and Shelly N Binkley and Mark Vandenbranden and Steven A. Wrighton},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={2001},
  volume={297 3},
  pages={1044-50}
}
The formation of R- and S-norfluoxetine was analyzed in vitro in human liver microsomes. Low apparent K(m) values for R-norfluoxetine formation of < or =8 microM and S-norfluoxetine of <0.2 microM were determined. R-Norfluoxetine formation rates in a characterized microsomal bank correlated with the catalytic activities for cytochrome P450 (CYP) 2D6, CYP2C9, and CYP2C8. Expressed CYP2C9, CYP2C19, and CYP2D6 formed R-norfluoxetine following incubation with 1 microM R-fluoxetine and exhibited… CONTINUE READING