Identification of the globin chain and the amino acid residue responsible for polymerization of bullfrog (Rana catesbeiana) hemoglobin with iodo[14C]acetamide.

@article{Goto1978IdentificationOT,
  title={Identification of the globin chain and the amino acid residue responsible for polymerization of bullfrog (Rana catesbeiana) hemoglobin with iodo[14C]acetamide.},
  author={Yoshio Goto and Takuya Tomizuka and Takumi Okazaki and R Shukuya},
  journal={Biochimica et biophysica acta},
  year={1978},
  volume={534 2},
  pages={374-7}
}
The bullfrog (Rana catesbeiana) major hemoglobin dissociates into its constituent globin chains (alpha and beta) which are separated by Sulfopropyl-Sephadex C-25 column chromatography after alkylation with iodo[14C]acetamide. Each globin chain has two cysteine residues and those of the beta-globin chain in the tetramer are preferentially alkylated with… CONTINUE READING