[Identification of the genome of a picornavirus isolate using Random-PCR].

Abstract

OBJECTIVE To identify the genome of an enterovirus isolate (XJ90). METHODS The double-stranded cDNA of isolated virus was synthesized with anchored-random primer and was amplified with anchor primer; the PCR products were cloned into pGEM-T vector for sequencing and analysis. RESULTS Analysis on agarose gel electrophoresis of the Random-PCR products showed a typical smear; 11 randomly selected clones were all homologous to the members of enteroviruses and the homology rate over 80%; the 11 clones were distributed evenly along the whole genome and they assembled 4 contigs totally 2 261 bp long which covered 30% of the genome. The homology of the fourth contig and coxsackie virus B6 was 97.3% at amino acids level. The virus gene was specifically amplified with primers derived from the sequenced clones. CONCLUSIONS The picornavirus isolate XJ90 is a member of enteroviruses.

Cite this paper

@article{Du2001IdentificationOT, title={[Identification of the genome of a picornavirus isolate using Random-PCR].}, author={Gui-xin Du and Wynn Hwai-Tzong Pan and Wen Chen and Hongtao Wang}, journal={Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology}, year={2001}, volume={15 3}, pages={242-4} }