Identification of the forms of insulin-like growth factor-binding proteins produced by human fibroblasts and the mechanisms that regulate their secretion.

@article{CamachoHubner1992IdentificationOT,
  title={Identification of the forms of insulin-like growth factor-binding proteins produced by human fibroblasts and the mechanisms that regulate their secretion.},
  author={Cecelia Camacho-Hubner and Walker H. Busby and Robert H. McCusker and Gabriela Wright and David Robert Clemmons},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 17},
  pages={11949-56}
}
Human fibroblasts secrete insulin-like growth factor-binding proteins (IGFBPs) that can modify insulin-like growth factor (IGF) I action. We have determined the molecular identities of three forms of IGFBPs that are secreted by human fibroblasts in vitro. Ligand blot analysis of fibroblast conditioned media revealed that the M(r) 43,000 and 39,000 forms were the most abundant, but that M(r) 31,000 and 24,000 forms were also present. An antiserum that was specific for IGFBP-5 reacted with the M… CONTINUE READING