Identification of the first archaeal arylsulfatase from Pyrococcus furiosus and its application to desulfatation of agar

@article{Jung2012IdentificationOT,
  title={Identification of the first archaeal arylsulfatase from Pyrococcus furiosus and its application to desulfatation of agar},
  author={Keong-Tsul Jung and Han-Woo Kim and Dong-ju You and Soo-Wan Nam and Byung-Woo Kim and Sung-Jong Jeon},
  journal={Biotechnology and Bioprocess Engineering},
  year={2012},
  volume={17},
  pages={1140-1146}
}
A gene encoding a putative arylsulfatase from the hyperthermophilic archaeon Pyrococcus furiosus was identified, cloned, and expressed as a fusion protein with a Sce VMA intein and chitin binding domain (CBD) residue. The gene (PF1345) from P. furiosus encoding a 35 kDa protein showed some similarity (17 ∼ 19%) with other arylsulfatases from the bacteria. The recombinant fusion arylsulfatase was overexpressed in E. coli and partially purified. Its molecular mass was estimated to be 90 kDa by… CONTINUE READING

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