Identification of the fire blight pathogen, Erwinia amylovora, by PCR assays with chromosomal DNA.

@article{Bereswill1995IdentificationOT,
  title={Identification of the fire blight pathogen, Erwinia amylovora, by PCR assays with chromosomal DNA.},
  author={Stefan Bereswill and Peter Bugert and Iris Bruchm{\"u}ller and Klaus Geider},
  journal={Applied and environmental microbiology},
  year={1995},
  volume={61 7},
  pages={2636-42}
}
Erwinia amylovora, the causative agent of fire blight, was identified independently from the common plasmid pEA29 by three different PCR assays with chromosomal DNA. PCR with two primers was performed with isolated DNA and with whole cells, which were directly added to the assay mixture. The oligonucleotide primers were derived from the ams region, and the PCR product comprised the amsB gene, which is involved in exopolysaccharide synthesis. The amplified fragment of 1.6 kb was analyzed, and… CONTINUE READING

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