Identification of the enzymatic active site of tobacco caffeoyl-coenzyme A O-methyltransferase by site-directed mutagenesis.

@article{Hoffmann2001IdentificationOT,
  title={Identification of the enzymatic active site of tobacco caffeoyl-coenzyme A O-methyltransferase by site-directed mutagenesis.},
  author={Laurent Hoffmann and St{\'e}phane Maury and Marc Bergdoll and Laurent Thion and Monique Erard and Michel Legrand},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 39},
  pages={36831-8}
}
Animal catechol O-methyltransferases and plant caffeoyl-coenzyme A O-methyltransferases share about 20% sequence identity and display common structural features. The crystallographic structure of rat liver catechol O-methyltransferase was used as a template to construct a homology model for tobacco caffeoyl-coenzyme A O-methyltransferase. Integrating substrate specificity data, the three-dimensional model identified several amino acid residues putatively involved in substrate binding. These… CONTINUE READING