Identification of the earliest B lineage stage in mouse bone marrow.

  title={Identification of the earliest B lineage stage in mouse bone marrow.},
  author={Y S Li and Robert H. Wasserman and Kyoko Hayakawa and Richard R Hardy},
  volume={5 6},

Lineage specification and plasticity in CD19− early B cell precursors

Three CD19− B cell precursor populations in mouse bone marrow identified using 12-color flow cytometry demonstrate progressive acquisition of B lineage potential and support an asynchronous view of early B cell development, in which B lineage specification initiates in the MLP/CLP stage, whereas myeloid potential is not lost until the pre-pro–B (Fr. A) stage.

B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells.

It is suggested that B-lineage commitment can occur before the expression of B220 and CD19, andGene expression analysis demonstrated that these lineage-restricted cells express B- lineage-associated genes to levels comparable with that observed in pro-B cells.

Fates of human B-cell precursors.

The triad of laboratory models that mimic the bone marrow microenvironment, immunodeficiency diseases with specific defects in B- cell development, and B-lineage acute lymphoblastic leukemia can now be integrated to deepen the understanding of human B-cell development.

The Earliest Step in B Lineage Differentiation from Common Lymphoid Progenitors Is Critically Dependent upon Interleukin 7

It is suggested that IL-7 plays a key and requisite role during the earliest phases of B cell development as well as in vitro differentiation of CLPs into B220+ CD19+ B lineage progenitors.

Functional characterization of B lymphocytes generated in vitro from embryonic stem cells.

An efficient system for the differentiation of embryonic stem cells into mature Ig-secreting B lymphocytes is established and the generation of A-MuLV transformants from ES cells will provide an advantageous system to investigate genetic modifications that will help to elucidate molecular mechanisms in V(D)J recombination and in A- MuLV-mediated transformation.

A subfraction of B220+ cells in murine bone marrow and spleen does not belong to the B cell lineage but has dendritic cell characteristics

The findings indicate that the mouse bone marrow contains a recirculatingpopulation of B220+CD19– CD11c+ plasmacytoid DC, the development of which is largely independent of the presence of mature T and B cells.

Identification of a germ-line pro-B cell subset that distinguishes the fetal/neonatal from the adult B cell development pathway

Findings demonstrate that the B-1 development pathway does not include the major germ-line pro-B subset found in adult BM and hence identify a very early difference between theB-1 and -2 development pathways.

Loss of c-kit accompanies B-lineage commitment and acquisition of CD45R by most murine B-lymphocyte precursors.

Analysis of B-lineage progression during a finite culture period showed that the most mature precursors were concentrated in the Lin-c-kit- population, suggesting that TdT expression is initiated as c-kit downregulation begins in Lin- cells, with progressive loss of c-Kit during B- lineage differentiation.